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Paulson, D.J
Group 3 ( n = 8) 16-h fasting mice
Patients with a history of pancreatitis should be monitored closely, and urgent medical attention should be sought for severe abdominal pain radiating to the back
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Briefly, dissected gonads were fixed in 3% paraformaldehyde/0.1M K 2 HPO 4 (pH 7.2) solution for 10-20 min, and then post-fixed with cold 100% methanol for 5 min (Alternatively, fixed worms can be stored in cold methanol at -20C for a few days)
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