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50 mg of tissue lysate was added to obtain a total of 50 L of the amidation assay buffer composed of: Catalase 100 ug/mL (freshly prepared), 2 mM L-ascorbic acid (freshly prepared), ZnCl 2 2 M, CaCl 2 2 M, CuSO 4 75 M, NAMES 100 mM, pH 5.5, 0.15 mM Dansyl-YVG