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Bacteria collected from 100 ml culture were lysed in 1 ml lysis buffer consisting of 858 l PBS, 40 l 25 complete protease inhibitor cocktail (Hoffmann-La Roche, Basel, Switzerland) 1 l 1 M DTT, 50 l 10 mg/ml lysozyme, 1 l 1 10 4 U/ml DNA ase I, and 50 l 20% Triton X on ice for 30~60 min, and then subjected to sonication

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DECLARATIONS Authors contributions Conceived the design of this article and wrote the first draft

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GuieuBLecouteyCLegayRDavisADe Oliveira SantosJSAltomareCDet al