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DG-75, BL-2, and Jurkat were cultured in RPMI 1640 medium (21875034), whereas HEK-293T were cultured in Dulbeccos modified Eagles medium (DMEM) (11965092), both from ThermoFisher Scientific (Waltham, MA, USA)

The formulas are often tailored for different stages in a womans life, such as the reproductive years, pregnancy, and menopause

Briefly, genomic DNA was extracted from the cell pellets and amplified using GenePrint System (Promega)

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