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Methods Cell culture and growth conditions All cell lines were cultured in either RPMI (Gibco, 11875119) or DMEM media (Gibco, 12430063) supplemented with 10% fetal bovine serum (Gibco, 12483020) and 1% penicillin-streptomycin (Gibco, 15140-122) and passaged by washing with Dulbeccos phosphate buffered saline (PBS) (Gibco, 14190250), and incubating with trypsin-EDTA (0.25%) (Gibco, 25200114)

doi:10.1007/s10495-017-1351-9 Nonaka H, Tsujino T, Watari Y, Emoto N, Yokoyama M

Then insert needle