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In fertility and early embryonic development studies, male and female rats were administered twice weekly subcutaneous doses of 0.5, 1.5, or 3 mg/kg (0.3-, 1-, and 2-fold and 0.3-, 0.9-, and 2-fold, respectively, the MRHD of 15 mg once weekly based on AUC)
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The salt-buffered gels were then added to 100 L reaction volumes containing a final concentration of 1X Superscript IV RT buffer, 5 mM DTT, 5 mM dNTPs, 5% ( v/v ) RNAase Inhibitor and 5% ( v/v ) Superscript IV reverse transcription enzyme and subjected to single cycle of reverse transcription (i.e
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