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The animals were placed in an induction chamber with an oxygen-enriched environment, with the initial induction concentration set at 4%5% isoflurane, until deep anesthesia signs such as loss of the righting reflex appeared, after which maintenance was initiated

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That said, it is important to note that a minority of studies using older, broad-spectrum NOS inhibitors have instead reported anxiogenic effects of NOS inhibition (Pitsikas, 2024)