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Using an ERE-luciferase mouse, a reporter mouse that allows monitoring of ER transcriptional activity in a spatiotemporal manner, it was demonstrated that estrogen signaling is locally activated in keratinocytes in vitro (with 1 nM E2) and in vivo (with 10 nM E2)

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Example 8 Table no 12: Formulation of batches using Carbopol Ultrez 10 Table no 13: Formulation of batches using Carbopol 971P NF Table no 14: Formulation of batches using Carbopol 974P NF Process of preparation of batches 1-11 is same as that described under example 2 for batch G