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If you have reconstituted with sterile water by mistake and cannot use the entire vial within 24 hours, the safest approach is to discard it and start fresh with bacteriostatic water

A.JayaramanM.MatsudaS.LiuJ.BarrosS.QuerbesW.et al (2021)

Purification of Polyhistidine-Tagged Proteins by FPLC The rigid particle structure of the silica base used in the HisLink Resin make this material an excellent choice for applications that require applied pressure to load the lysate, wash or elute protein from the resin

The other thing is inflammation