When two iron chelators, VK28 and VAR10303, were applied to the rd10 mouse model, it could be observed that the survival rate of photoreceptor cells, such as rod cells, was significantly improved, which greatly improved the visual function of the mice (115)
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To initiate a peroxidase reaction, the sections were rinsed with a washing buffer and subsequently incubated with 3,3-diaminobenzidine tetrahydrochloride (DAB, Thermo Fisher Scientific, Rockford, IL, USA)
Spacing injections seven days apart maintains steady-state levels in the body, reducing the fluctuations that more frequent dosing would create