Fluorescence (ex 545 nm/em 600 nm) was recorded with a microplate reader (CLARIOstar, BMG)
Cochrane Database of Systematic Reviews [Internet]
392 Likewise, LDH inhibitors contribute to photothermal therapy (PTT)
Regulatory guidance from the Food & Drug Administration (FDA) and WHO emphasizes that proper aseptic technique, storage procedures, environmental controls, and documentation practices remain essential regardless of the presence of preservatives
Then, primary antibody binding was detected after incubating sections with a horseradish peroxidase conjugated-secondary antibody (polyclonal anti-goat or anti-rabbit IgG HRP, R&D System, Minneapolis, MN, USA) for 2.5 h, followed by a 20 min exposure to the HRP substrate 3-amino-9-ethylcarbazole (AEC)