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10.3389/fphar.2021.622262 11 BakrisG

In different cell types, activation of necroptosis can be triggered by death receptors of the TNF family, including TNF receptor superfamily member 1A (TNFRSF1A/TNFR1), FAS, and TNF-related apoptosis-inducing ligand (TRAIL) receptors (TNF receptor superfamily member 10a [TNFRSF10A/TRAILR1] and TNF receptor superfamily member 10b [TNFRSF10B/TRAILR2]), that typically induce apoptosis

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Protein expression was induced with 1 mM IPTG at an OD of 0.60.8, followed by incubation at 20 C for 5 h