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After 24 h, migrated cells on the upper surface of the filter were removed with a cotton swab, while the cells on the bottom of the filter were fixed with 4% paraformaldehyde at room temperature for 15 min and stained with 3% crystal violet solution for 15 min

Chen BY, Pathak JL, Lin HY, Guo WQ, Chen WJ, Luo G, et al

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Scientists claim that topical products act locally and generally have a low systemic absorption rate, which limits their ability to compromise the fetus

That said, the evidence suggests that 6mg remains a reasonable option for many people