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This allowed us to isolate the effect of prolonged stress on fear extinction, demonstrating that PTSD-model mice (fear conditioning + SPS) exhibited impaired extinction retrieval compared to the footshock-only group, despite acquiring similar levels of fear during conditioning

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In the CNS, melatonin is metabolized mainly by the enzyme indoleamine 2,3-dioxygenase (IDO), giving rise to the metabolite N1-acetyl-N2-formyl-5-methoxykynuramine (AFMK), which is subsequently transformed into N-acetyl-5-methoxykynuramine (AMK) [1,6]