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Washed beads were resuspended in 100 L of 50 mM HEPES buffer (pH 7.4) and sequentially reduced with TCEP (200 M) and re-alkylated either with IAM (400 M) Procedure A or with IAM-TPP (400 M) Procedure B for 30 min at RT in the dark

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Those who experienced increased levels of anxiety reported reduced QoL in 6/8 measured domains

The combination of DNase-I + amikacin resulted in a strong increase in dead biofilm compared to DNase I treatment alone, and the triple therapy combination of GSH + DNase-I + amikacin showed the highest level of biofilm disruption (Figure 3C)