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It's incorrect dilution math that renders an entire vial useless before the first injection

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Relative quantification of gene transcription The response to nitrogen availability at the mRNA level of genes encoding for GS ( glnA1 ), NADP + -GDH ( msmeg_5442 ) and the L_180 NAD + -GDH ( msmeg_4699 ) was assessed by semi-quantitative Real-Time PCR [48]

2010 Apr;151(4):1473-86 Madsen LW, Knauf JA, Gotfredsen C, Pilling A, Sjgren I, Andersen S, Andersen L, de Boer AS, Manova K, Barlas A, Vundavalli S, Nyborg NC, Knudsen LB, Moelck AM, Fagin JA