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Optimization of HPLC-MS/MS conditions All experiments were conducted using an Agilent 1290 Infinity II liquid chromatographic system (Agilent, Palo Alto, CA, USA) equipped with a quaternary high-pressure pump, a thermostated autosampler, a thermostated column compartment, and a diode array UV detector

present in the biological samples and also minimizes leaching of affinity ligands from the matrix

The cell pellet was then collected under 4 C and stored at 80 C

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