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Nuclear-to-Cytoplasmic (N/C) Ratio Quantification The N/C ratio of NRF2 was calculated by dividing the mean fluorescence intensity (MFI) of NRF2 in nuclei (DAPI-defined regions) by that in cytoplasm (-tubulin-outlined areas excluding nuclei), using ImageJ Software (Version 1.53c)

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After deparaffinization, sections were heated in an autoclave at 121 C for 5 min in 10 mM sodium citrate buffer (pH 6.0) or reacted with protease solution (Nichirei Biosciences, Tokyo, Japan) for epitope retrieval, after which endogenous peroxidase was inactivated by 0.3% hydrogen peroxide in methanol

In a chronic corticosterone-induced depression mouse model, liraglutide reduced depressive and anxious behaviors, mitigated corticosterone-induced hyperactivity, and preserved synaptic plasticity, unlike fluoxetine, which failed to alleviate hippocampal long-term potentiation suppression (Weina et al