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PLoS ONE 89 YaoJ

2024;7(1):e2350408

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As miR-194-3P is responsible for progesterone resistance [119] and apigenin can modulate progesterone receptor [175], hence, future research unveiling the effect of apigenin on miR-194-3P will explain its mechanistic pathway in a better way

coli containing pET-28b(+) based plasmids (pANT13-16, pANT18, pANT20-21, pANT35, pANT95, pJK1-3, pJK7-9 or site saturation variants of pANT35) were inoculated from an overnight preculture at a ratio of 1:100 in 96-deep-well plates equipped with a CR1296 sandwich cover (EnzyScreen) in 0.5 ml TB medium containing 50 g ml 1 kanamycin sulfate per well and incubated at 37 C, 300 rpm using a 5 cm shaking diameter for 2.5 h