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The lysate was clarified by centrifugation at 15,000 nx g for 15 min, protein concentrations were determined using the BCA total protein assay (Pierce), and then appropriate volumes of the lysates were diluted with 2x reducing Laemmli buffer and heated for ten min at 95 C

While gastrointestinal side effects such as nausea and vomiting are most common with these medications, some patients also experience heartburn and gastro-oesophageal reflux symptoms

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Analysis of endoscopic brush samples identified mucosa-associated dysbiosis in inflammatory bowel disease

Understanding your genetic profile can help your provider anticipate tolerability patterns and tailor dosing strategy to minimize side effects while maximizing therapeutic benefit