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The mRNA levels were measured employing SYBR Green PCR premix (TaKaRa, Japan)

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While the association between reactive oxygen species (ROS) and antioxidants in humans is usually well balanced to maintain normal metabolic activity, excessive ROS generation or decreased antioxidative activity resulting from any cause may lead to oxidative stress and thereby induce adverse effects and diseases, including cancer (Milkovic et al., 2014)

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