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As a result, this section focuses on direct binding and interaction of semaglutide to GLP-1R ECD to ensure that the semaglutide analogue reaches GLP-1R ECD faster, binds to GLP-1R ECD stronger (K d ) and faster (K on ), and dissociates from GLP-1R ECD slower (K off ), compared to native semaglutide reaching and binding to GLP-1R ECD [29]

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