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Bezwada, D

Hathroubi et al

In mammalian cells, glutamine is a principal energy substrate

Possible side effects associated with overconsumption of vitamin C include kidney stones, nausea, and diarrhea ( The U.K.s National Health Service (NHS) only advises that vitamins and mineral supplements be given to infants 6 months of age or older (4)

On day 6, cells were counted, and 100 l cell suspension (in DMEM media supplemented with only 10% FBS but no antibiotics) was added to the 96-well plates (containing siRNA and transfection mix) at the appropriate seeding density (12k for DMSO and 15k for etoposide-treated cells)