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Step 4: Schedule follow-up labs

For imaging, all cells were incubated in staining solution at room temperature and then moved to a 37 C incubator

AMPK activation subsequently inhibits the mTORC pathway (a major regulator of cell growth and proliferation) and promotes cell cycle arrest by stabilizing p53 and the p21/p27 inhibitors ( Preclinical studies validate these direct anti-tumor effects across different agents: Liraglutide, for instance, demonstrates potent anti-cancer activity by suppressing HCC progression in mouse models, crucially exhibiting an anti-inflammatory effect by inhibiting the IL-6/STAT3 signaling pathway (Kojima et al., 2020

This real-world evidence provides a validation of the oral semaglutide data used in the present analysis taken from PIONEER 4, but could not be used to inform a full analysis, as no liraglutide arm was included and use of these treatment effects in a different baseline population is potentially inappropriate