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Of the cohort, 72.8% had established CVD, 16.3% had CV risk factors and 10.9% had chronic kidney disease with no prior CVD

Growth at 37 C with 230 rpm shaking in LB-Lennox medium (Difco) was used for routine strain propagation for all strains, unless otherwise specified

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Here are the main causes: Psychological Factors Stress and anxiety Depression Relationship issues Performance Anxiety Biological Factors Cardiovascular diseases like heart disease, high BP Type 2 diabetes Obesity Hormonal imbalances like low testosterone (low T) Neurological disorders like multiple sclerosis Lifestyle Influences Poor diet Lack of exercise Smoking & drug use Excessive alcohol consumption Medications Certain prescription drugs, such as antidepressants and antihypertensives Understanding the causes of erectile dysfunction is the first step toward effective treatment