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Subsequently, the blocked PVDFs were reacted with the appropriate primary antibody diluted in BSA blocking buffer (5% bovine serum albumin in 13.7 mM NaCl, 0.27 mM KCl, 0.2 mM KH 2 PO 4 , 1 mM Na2HPO 4 , 0.1% Tween 20) overnight at 4 o C

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