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Laboratory parameters assessed at baseline and at 6 months included liver function tests [aspartate aminotransferase (AST), alanine aminotransferase (ALT), and -glutamyltransferase (GGT)], lipid profile [total cholesterol, low-density lipoprotein (LDL) cholesterol, high-density lipoprotein (HDL) cholesterol, and triglycerides], HbA1c, and FBG

Starting protocols are usually small (around 250 mcg) and increased gradually, so per-dose draws are small and benefit from a U-100 insulin syringe for readability

After being washed three times with PBS, the cells were observed and imaged under a fluorescence microscope (Nikon, Japan)