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The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification and purification

Food and Drug Administration (FDA) or any other regulatory agency for any specific purpose

GLP-1 receptor agonists reduce appetite and slow gastric emptying, creating a caloric deficit that leads to fat mobilisation throughout the body

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Analysis was conducted in three separate batches, with QC samples analysed in triplicate at the start, middle, and end of each batch to assess between-batch reproducibility, with RSD% calculated for each analyte