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All cell lines were treated with a fixed dose of MTX (25 nM) for 24 hours, as this dose was representative of MTXs average IC50 at 96 hours across all cell lines (ie, 18.4 nM)

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Human p53 is inhibited by glutathionylation of cysteines present in the proximal DNA-binding domain during oxidative stress

Hallmann, L., Ulmer, P., Reusser, E., Louvel, M