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About this article Cite this article Janardhanan, M., Rakesh, S., Savithri, V

In a cortical spreading depression (CSD) model [13, 14], which mimics the aura of migraine, and the chronic inflammatory soup (IS)-triggered CM model, microglia are dramatically activated [15, 16]

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Cells were seeded at a density of 5000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment