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HbA1c was measured by high-performance liquid chromatography (HPLC)

To reduce the number of steps in a sample preparation procedure a simultaneous reduction of disulfide bound with tris(2-carboxyethyl)phosphine (TCEP) and the selective pre-column derivatization of the thiol group with 1-benzyl-2-chloropyridinium bromide (BCPB) has been applied

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