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Figure 14a shows an increased expression (immunofluorescence) of GSTA4-4, an oxidative stress specific GST isoenzyme, in HepG2 cells after ASA treatment.NAC treatment brought the enzyme activity close to control values in 5 mol/ml ASA treated cells but not in 10 mol/ml ASA treated cells, where the enzyme expression remained significantly higher
However, amino acid analogs are unsuitable for clinical use due to their low affinity, lack of specificity, and toxicity 87
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