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Angle the needle and slowly allow water to slide down and gently contact the lyophilized material

The exosomes obtained were resuspended in PBS for subsequent experiments

Baseline labs, including hormone tests, are invaluable during transitions such as perimenopause

Strains for further studies were stored in the MAST CRYOBANK system (Mast Diagnostic, United Kingdom) at 80 C

Cells were seeded at a density of 5000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment