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Bae, S.H., et al.,Sestrins activate Nrf2 by promoting p62-dependent autophagic degradation of Keap1 and prevent oxidative liver damage
This method ensures a precise concentration, facilitating accurate dosing for research purposes
ELISA Kit Components Required Instruments and Reagents Microplate reader (wavelength: 450nm) 37C incubator (CO2 incubator for cell culture is not recommenced.) Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose) Precision single (0.5-10L, 5-50L, 20-200L, 200-1000L) and multi-channel pipette with disposable tips(Calibration is required before use.) Sterile tubes and Eppendorf tubes with disposable tips Absorbent paper and loading slot Deionized or distilled water Assay Procedure Summary Step 1: Add 100ul standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37C
Lipoic acid: Women with polycystic ovary syndrome who supplemented with lipoic acid showed an improvement in insulin sensitivity and a reduction in triglycerides, while improvements in menstrual regularity were also seen with a combination of lipoic acid and inositol
