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The 2025 Frontiers in Aging clinical review (Frontiers in Aging 2025) summarises the documented short-term adverse effects in adult populations: peripheral oedema and fluid retention are common at initiation, carpal tunnel syndrome occurs in a meaningful proportion of users, hyperglycaemia and insulin resistance can emerge because GH is physiologically counter-regulatory to insulin, and gynaecomastia has been reported, particularly at higher doses

Briefly, slides were immersed in 4% paraformaldehyde (Thermo Fisher Scientific, catalog: J19943.K2) immediately after removal from the freezer for 1 min, then washed with water

Intravenous glutathione bypasses absorption barriers and is used clinically at doses of 6002400 mg per administration, often once or twice weekly