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J Neurochem 2011

In DKD, continuous activation of RAAS leads to elevated pressure within the glomeruli, enhances the production of ROS, promotes tissue damage, triggers the release of proinflammatory cytokines, and stimulates the development of fibrosis in the glomeruli and tubulointerstitium

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Derivatized protein samples were then reduced in 8 M urea, 15 mM dithiothreitol (DTT) at 60 C for 40 min, and then alkylated with 35 mM iodoacetamide (IAM) at room temperature in the dark for 40 min