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The assessment on the effects of the in vitro culture media composition, including carbon and nitrogen sources, pH, and temperature are all necessary for enhancing mushroom mycelial biomass growth and production

Melo G, Duarte J, Pauletto P, et al

Clinical trials showed that GLP-1 agonists improved blood sugar control while also causing weight loss as an unexpected side effect, which intrigued researchers exploring metabolic therapies

To generate lentiviral constructs, the cDNA of WT or mutant ASPL was amplified using Phusion high-fidelity DNA polymerase and cloned into an N174 vector (Addgene, #31822) using the standard restriction digestion/ligation procedure