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All mouse experiments were performed in compliance with the guidelines of the Institutional Animal Care and Use Committee of Southern Medical University Nanfang Hospital and were approved under protocol number IACUC-LAC-20220926-001

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Senescent erythrocytes are characterized by loss of surface area and volume [66], increased cell density [67], reduced deformability [68], cation loss [69], membrane desialylation [70], enzymatic dysregulation [69], oxidative stress [71], hemichrome abundance [69], elevated levels of glycated hemoglobin [72], reduced expression of phagocytosis-inhibiting CD47 [73], altered protein 4.1a/protein 4.1b ratio [74], etc