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Purified RNA was reverse-transcribed into cDNA using the First Strand cDNA Synthesis kit (K1622, Thermo Fisher Scientific, USA) according to the manufacturer's protocol

During these check-ins, your provider will: Youll also have 24/7 access to medical support for any questions or concerns

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These core pathophysiological components exhibit intricate bidirectional crosstalk, operating through mutually reinforcing mechanisms that collectively perpetuate the syndromes phenotypic heterogeneity and clinical progression ( Figure 1 )