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After first centrifugation, plasma and buffy-coat layer were removed, and RBCs were washed twice by centrifugation at 400 g for 3 min (Centrifuge ELMI-50CM, Riga, Latvia) and resuspended in HEPES-buffer

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For reticulin staining, slides were immersed in potassium permanganate solution, followed by sulfuric acid, oxalic acid, ferric ammonium sulfate solution, silver nitrate solution, formaldehyde solution, gold chloride solution, and sodium thiosulfate solution (#1.00251, Sigma-Aldrich, St