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Healthy endothelial cells rely on an enzyme called endothelial nitric oxide synthase (eNOS) to produce nitric oxide (NO)

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For instance, NKCC2 has been found in insulin-secreting -cells (Zhu et al., 2011) or neurons of the hypothalamus (Konopacka et al., 2015), whereas NCC was detected in endothelial and smooth muscle cells, heart, lung and liver (Wang et al., 2015), adipocytes (Zhang et al., 2022a), and -cells as well (Zhang et al., 2022b)

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HEK-293T cells were transfected with packaging and target plasmids using Lipofectamine 3000 (Thermo Fisher Scientific)