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The American Academy of Ophthalmology has not recommended that patients stop using semaglutide, but rather, that patients experiencing vision loss while taking semaglutide should stop the drug and contact the physician prescribing the medication

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Podlesny D, Durdevic M, Paramsothy S, et al

For immunoprecipitation, cell lysates were prepared as before and rotated 1 mg proteins with antibody at 4 C overnight, then immunoprecipitated by Protein A/G beads