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After three washes with TBST, membranes were incubated with goat anti-rabbit or mouse secondary antibody (1:5000) at room temperature for 1 h, followed by three 10-min washes

A final concern is that many dietary supplements products simply arent composed of what they purportedly contain

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Fusion activity was higher with elastase alone than with trypsin alone for both RaTG13 and Khosta-2 spikes, suggesting that elastase may act as a more efficient fusion trigger than TMPRSS2

Y.LiuY.et al (2010)