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Standard good laboratory practice (GLP) diet and fresh water were provided ad libitum

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According to NHS guidelines: Below 200 ng/L (148 pmol/L) Generally indicates deficiency Below 100 ng/L (75 pmol/L) Usually shows clinical or metabolic evidence of deficiency Most healthy people have levels around 500 ng/L However, its worth noting that some people experience symptoms even with B12 levels in the normal range, particularly older adults