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After solubilization of neutral-red stain with 0.1 mL of acidified alcohol (10% acetic acid, 40% ethanol in distilled water) the absorbance was measured in a microplate reader at 550 nm

Marso SP, Daniels GH, Brown-Frandsen K, Kristensen P, Mann JF, Nauck MA, Nissen SE, Pocock S, Poulter NR, Ravn LS

The exact mechanism of how Ozempic causes weight loss is not fully understood, but it is believed to be related to the medications effects on the brains appetite control center

Summary: Wegovy may not work effectively due to insufficient treatment duration (requires 16+ weeks to reach maintenance dose), inadequate lifestyle modifications, poor medication adherence, underlying medical conditions such as hypothyroidism, or concomitant medications that promote weight gain

653 Martin-Taboada, M