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Pro-oxidants: fresh hydrogen peroxide (Sigma, UK) solution was prepared as a 10 mM stock solution immediately before use then diluted to the required concentration

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It is important to note the regions of the brain that are more susceptible to the changes in BBB integrity as regions like the hippocampus and amygdala are heavily affected in neurodegenerative disease as they are vital for long-term memory and processing emotions and behavior