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Negative binding energy values mean that the interaction between the ligand and the protein is thermodynamically favourable
Complex IV (cytochrome c oxidase) was assayed by monitoring the oxidation of reduced cytochrome at 550 nm 16
Moreover, liver enzyme activities, including alanine aminotransferase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP), were determined using the methods described by 44,45 , and immunoglobulins G, including immunoglobulin A (IgA), immunoglobulin M (IgM), and immunoglobulin G (IgG) values were determined using ELISA kits according to 46
8b, c) to avoid a futile cycle due to the nonenzymatic shortcut (Fig