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After 30 minutes, the media was replaced with fresh serum-free media and incubated for further 24 hours

By session two, the technique is routine

[DOI] [PMC free article] [PubMed] [Google Scholar] 23.Barisic I., Balenovic D., Udovicic M., Bardak D., Strinic D., Vlainic J., Vranes H., Smoday I.M., Krezic I., Milavic M., et al

AOD9604 is a 16-amino acid fragment corresponding to positions 176-191 of the human growth hormone sequence, isolated for its lipolytic activity while being structurally distinct from the growth-promoting domain

The active ingredients reach the systemic circulation faster and without reduction in concentration compared to the delivery through the gastrointestinal tract, resulting in a stronger effect