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Clinically, late-phase pain often becomes dissociated from inflammation and resistant to anti-inflammatory therapies, resembling NPP

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An Ultimate 3000 UHPLC (Dionex) was coupled to the Q Exactive-Mass plus spectrometer (QE-MS, Thermo Scientific) for metabolite separation and detection

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Documented symptoms include precocious (early) puberty, accelerated growth, unresolved virilization, and female infertility