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Western blotting Antibodies used in cellular studies include anti-p-p70 S6K (Thr389) (1A5) (mouse mAb, 9206, 1:1,000 for western blotting), anti-p70 S6K (rabbit, 9202, 1:1,000 for western blotting), anti-4E-BP1 (rabbit, 9644, 1:1,000 for western blotting), anti-p-4E-BP1 (Ser65) (rabbit, 9456, 1:1,000 for western blotting) and anti-RAPTOR (24C12) (rabbit, 2280, 1:1,000 for western blotting) from Cell Signaling Technology

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